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Simple High-Performance Liquid Chromatographic Method for Simultaneous Determination of Fusidic Acid and Betamethasone Dipropionate in a Cream Formulation

Author(s):  Peh Kok-Khiang, Tan Yvonne Tze-Fung

Issue:  May/Jun 2000 - Compounding for the Ear, Nose and Throat
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Abstract:  A simple and selective high-performance liquid chromatography (HPLC) method using ultraviolet detection was developed for simultaneous determination of fusidic acid and betamethasone dipropionate in a cream formulation. A Supelcosil® L C18 column was used for chromatographic separation. The mobile phase consisted of acetonitrile and 0.01 M disodium hydrogen orthophosphate (70:30, % v/v) adjusted to pH 6 with glacial acetic acid. Analysis was run at a flow rate of 1.0 mL/minute with the detector operating at 235 nm. The standard calibration curve was linear over a concentration range of 0.3 to 1.2 mg/mL for fusidic acid and 9.6 to 38.4 µg/mL for betamethasone dipropionate. The average recovery values for fusidic acid and betamethasone dipropionate were almost 100%. The within-run and between-run coefficient of variation and percent error values for the two drugs were all less than 2% and ± 3%, respectively.

Related Keywords: acetonirile, anti-inflammatory, assay, betamethasone, chromatogram, chromatography, fusidic acid, High-Performance, HPLC, liquid chromatography, method, spectrophotometer, steroids, supelcosil

Related Categories: PEER-REVIEWED, STABILITIES, COMPATIBILITIES

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