LP3 Network Free Webinar - Veterinary Compounding, A Customized Approach

Download in electronic PDF format for $25



Search the Complete Journal Archives

Quantitation of Testosterone in Suspension using High Performance Liquid Chromatography

Author(s):  Gupta Vishnu D

Issue:  May/Jun 1999 - Compounding for Animals
View All Articles in Issue

Abstract:  An assay method based on high-performance liquid chromatography (HPLC), for the quantitation of testosterone in an aqueous suspension, has been developed. The method is very simple compared with a tedious and time-consuming method recommended by the USP-NF. It is an accurate and precise method; the percent relative standard deviation based on five injections was 1.15. The average recovery from four batches of suspension was 100% (percent relative standard deviation, 1.3). A number of inactive ingredients present in the suspension vehicle did not interfere with the assay procedure. Testosterone appears to be a very stable drug since it did not decompose significantly, even on heating. A 15-cm column gave sharper peaks vs. a 25-cm column and the sensitivity of the assay method increased by 400%.

Related Keywords: high performance liquid chromatography, HPLC, aqueous suspension

Related Categories: HRT, PEER-REVIEWED, STABILITIES, COMPATIBILITIES

Printer-Friendly Version

Related Articles from IJPC
Title (Click for Abstract / Details) Author Issue Page View/Buy
Quantitation of Testosterone in Suspension using High Performance Liquid Chromatography Gupta Vishnu D May/Jun 1999 239-240 Buy
Quality-Control Analytical Methods: High-Performance Liquid Chromatography Kupiec Thomas C May/Jun 2004 223 Buy
Quantitation of Testosterone Propionate in an Injection Using High Performance Liquid Chromatography Gupta Vishnu D Mar/Apr 1999 158-159 Buy
Quantitation of Testosterone-Cypionate-in-Oil Injection using High Performance Liquid Chromatography Gupta Vishnu D, Pramar Y Jul/Aug 1999 314-315 Buy
Stability of an Oral Liquid Dosage Form of Trovafloxacin Mesylate and Its Quantitation in Tablets Using High-Performance Liquid Chromatography Gupta Vishnu D May/Jun 2000 233-235 Buy
Simple High-Performance Liquid Chromatographic Method for Simultaneous Determination of Fusidic Acid and Betamethasone Dipropionate in a Cream Formulation Peh Kok-Khiang, Tan Yvonne Tze-Fung May/Jun 2000 229-231 Buy
Preparation and Characterization of a Compounded Aqueous Human Insulin Suspension Al-Achi Antoine, Parekh Bhavita Nov/Dec 2004 492-495 Buy
A New High-Performance Liquid Chromatographic Method for Fludarabine and Fludarabine Phosphate Compounded in Liposomes Abang Anthony M, Abbas S Ali, Pham Trinh, Lambros Maria Polikandritou Jul/Aug 2001 320-322 Buy
Quality-Control Analytical Methods: Gas Chromatography Kupiec Thomas C Jul/Aug 2004 305 Buy
Extemporaneous Formulations: How to Evaluate HPLC Stability Studies Chan Debora Schotik Nov/Dec 1999 447 Buy