LP3 Network Free Webinar - Veterinary Compounding, A Customized Approach

Download in electronic PDF format for $25



Search the Complete Journal Archives

Quality-Control Analytical Methods: High-Performance Liquid Chromatography

Author(s):  Kupiec Thomas C

Issue:  May/Jun 2004 - Andropause
View All Articles in Issue

Abstract:  Chromatography is an analytical technique based on the separation of molecules due to differences in their structure and/or composition. In this article, the author discusses practical aspects of high-performance liquid chromatography theory (chromatographic principles such as retention, resolution and sensitivity), chromatographic mechanisms, instrumentation, mobile phase and reservoir, pumps, injectors, detectors, data-acquisition/display systems and columns, separation techniques (isocratic vs. gradient elution and derivatization), quantitative analysis (external standard and internal standard) and validation. Figures show a chromatogram of a reference standard and a sample. High-performance liquid chromatography is currently the most widely used method of quantitative analysis in the pharmaceutical industry and in pharmaceutical analytical laboratories.

Related Keywords: High-performance liquid chromatography, quality-control analytical methods for, HPLC, quality-control analytical methods for, Kupiec, Tom, PhD, Quality-control analytical methods, for high-performance liquid chromatography

Related Categories: BUSINESS, SUPPORT, QUALITY CONTROL

Printer-Friendly Version

Related Articles from IJPC
Title (Click for Abstract / Details) Author Issue Page View/Buy
Quality-Control Analytical Methods: High-Performance Liquid Chromatography Kupiec Thomas C May/Jun 2004 223 Buy
Quality-Control Analytical Methods: Gas Chromatography Kupiec Thomas C Jul/Aug 2004 305 Buy
Quality-Control Analytical Methods: Selecting an Analytical Method Allen Loyd V Jr Jan/Feb 2004 61 Buy
Quality-Control Analytical Methods: Specific-Gravity Determinations of Liquids and Semisolids Allen Loyd V Jr Nov/Dec 2003 477 Buy
Quality-Control Analytical Methods: Osmolality and Osmometry Allen Loyd V Jr Mar/Apr 2004 143 Buy
Quality Control Analytical Methods: Method Validation Klang Mark G, Williams LaVonn A Sep/Oct 2016 381-386 Buy
Quality-Control Analytical Methods: Glossary of Quality Control/Quality Assurance Terms in Pharmaceutical Compounding Kupiec Thomas C, Okeke Claudia, Allen Loyd V Jr, Denison Clay Jul/Aug 2005 300-302 Buy
Quality-Control Analytical Methods: Viscosity Measurements Allen Loyd V Jr Jul/Aug 2003 305 Buy
Quality-Control Analytical Methods: In-House Quality Control of Simple Compounds Trusley Craig Nov/Dec 2007 487-490 Buy
Quality-Control Analytical Methods: Designing a Quality-Control Potency Testing Program for a Compounding Pharmacy Zolner William J Sep/Oct 2009 412-418 Buy